Objective To investigate the physical and functional relation between ERɑ and p73ɑ in breast cancer cells.Methods Protein expressions of ERɑ and p73ɑ were examined by Western Blot.The binding assay for ERɑ and p73ɑ was tested with Co-IP and immunoblot.Transcriptional activity of ERɑ and p73ɑ was analysed by Luciferase assay.Results and Conclusion(1)ERɑ and p73ɑ can bind to each other.(2)ERɑ and p73ɑ can be combined to form complex and mutually inhibit protein expression of each other in breast cancer cells.

<正>乳腺癌是女性最常见的恶性肿瘤,其发病率占全身恶性肿瘤的7～10%,近年来其发病率逐年上升,且呈年轻化趋势[1],而受到人们的广泛关注。由于分子生物学和免疫组化技术的发展,人们逐渐考虑从基因水平上探讨其发生机制以及从基因水平上治疗肿瘤,而p53基因与癌症的发展关系密切,p53基因与人类50%的肿瘤有关。人类肿瘤中常有p53基因