【Objective】The published SSR primers of cowpea,common bean and EST-SSR(expressed sequence tags) primers of common bean were located and integrated in azuki bean linkage group to construct a linkage map of Chinese azuki bean with azuki bean SSR primers as anchor markers.More practical molecular markers will be supplied to gene mapping,cloning and molecular marker-assisted selection of azuki bean.【Method】 A total of 1 473 SSR and EST-SSR primer pairs,including 906 SSR primer pairs of cowpea,123 SSR and 248 EST-SSR of common bean,196 SSR primer pairs of azuki bean,were used for PCR amplification to screen polymorphic markers between cultivated azuki bean parents of HB801×AG109 and GM892×AG110.Their F2 segregating populations were tested with the polymorphic markers.【Result】 An integrated genetic linkage map of azuki bean containing 145 SSR and EST-SSR markers was constructed.This molecular genetic linkage map is composed of 59 azuki bean SSR anchoring markers,newly mapped 63 cowpea SSR markers,9 common bean SSR markers and 14 common bean EST-SSR markers,as well as a stem colour marker-the purple stem trait which is located on linkage group 9.Genetic distance of purple stem trait from CEDG022 and cbess058 molecular markers is 0.9 cM and 0.1 cM,respectively.The total length of the linkage map was 823 cM and covered 11 linkage groups.The average distance between markers was 5.64 cM.The average distance of each linkage group spanned74.82 cM.The average number of markers was 13.27 for each of 11 chromosomes.The length of each linkage group ranged from 7to 26 markers was from 49.1 cM to 125.6 cM.【Conclusion】Molecular markers of the relative species were freshly introduced in azuki bean and increased density of its genetic linkage map.It is useful for gene mapping and cloning,molecular marker-assisted selection in azuki bean.

为了进行小豆种质创新和获得基因定位和克隆及其基因功能分析的突变体材料,分别用30、35、40、45、50、55Gy剂量的快中子,辐照小豆‘京农6号’干种子。M1出苗率分别为31.72%、29.06%、30.29%、36.51%、16.15%和15.17%,变异率为3.20%、4.70%、6.75%、6.61%、4.00%和2.20%。辐射剂量增加,出苗率降低、变异率呈增加趋势,M2代共获得77份叶形、株型、粒型、籽粒大小、粒色、叶色、早熟、丰产等性状变异材料。40～45Gy为小豆快中子诱变的适宜剂量,这些突变体为小豆相关基因定位克隆、功能分析和育种提供了有价值的材料。