Objective: To investigate the role and mechanism of long non-coding RNA (lncRNA) C9ORF139 targeting micro RNA(miR)-24-3P/TAOK1 in regulating the proliferation of acute myeloid leukemia (AML) cells. Methods: AML cells HL-60 and THP-1 were purchased from the Chinese Academy of Sciences and divided into 4 groups:group A was negative control group (siNC group), group B was interference C9ORF139 group (siC9ORF139 group), group C was siC9ORF139+miR-24-3p inhibitor group, and group D was miR-24-3P+TAOK1 overexpression group (oe-TAOK1 group). Real-time fluorescence quantitative reverse transcription PCR was used to detect the expression levels of AML cell lines of HL-60 and THP-1 in four groups. Cell Counting Kit-8 assay was performed to measure cell proliferation. Flow cytometry was applied to analyze cell apoptosis. Transwell test was applied to detect cell migration and invasion ability. Western blot was used to detect p-serine/threonine kinase (p-raf) and p-mitogen activation proteinkinase (p-MEK), p-extracellular regulatory protein kinase (p-ERK) expression. The luciferase reporter gene plasmid was constructed to verify the binding ability of C9ORF139,miR-24-3P and TAOK1.Nude mice were inoculated with subcutaneous tumor cells of HL-60 (group A) and HL-60 (group B). Results: After the C9ORF139 gene was knocked down and cultured for 120 h, The cell proliferation ability (0.62±0.02, 0.82±0.02), migration ability (0.22±0.03, 0.05±0.01), invasion ability (0.20±0.02, 0.13±0.03) of group B were all lower than that of group A (1.30±0.02, 1.83±0.07; 0.99±0.02, 0.99±0.02; 1.00±0.01, 1.00±0.01) (all P<0.05). When co-transfected with miR-24-3 inhibitor, cell proliferation ability, migration ability and invasion ability were all higher in group B (all P<0.05). When co-transfected with miR-24-3P and oe-TAOK1 plasmid, cell proliferation ability, migration ability and invasion ability were all higher than group B (all P<0.05).When the C9ORF139 gene in the cells was knocked down, the apoptosis level of group B (28.56±8.07, 17.74±1.91) were higher than those of group A (0.31±0.27, 2.49±0.33)(all P<0.05); when co-transfected with miR-24-3P inhibitor, the apoptosis level (2.34±0.09, 3.06±0.06) were lower than those in group B (all P<0.05); when co-transfected with miR-24-3P and oe-TAOK1 in the plasmid group, the apoptosis level (2.16±1.29, 4.80±0.37) were also lower than those of group B (all P<0.05). In HL-60 and THP-1 cells, when C9ORF139 was not mutated, the luciferase activity of miR-24-3P group was lower than that of the miR-NC group (P<0.05). When the binding site with miR-24-3p in C9ORF139 sequence was mutated, the luciferase activity in miR-24-3p group was equivalent to that in miR-NC group (P>0.05).When TAOK1 was not mutated; the luciferase activity of miR-24-3P group was lower than that of group A (P<0.05). When the binding site with miR-24-3p in TAOK1 sequence was mutated, the luciferase activity in miR-24-3p group was equivalent to that in miR-NC group (P>0.05).When the C9ORF139 gene in HL-60 cells was knocked down and cultured for 72 h, the phosphorylation expression levels of Raf, MEK and ERK molecules in group B were significantly lower than those in group A (all P<0.05). By day 14, the tumor volume in the group A was greater than the tumor cell volume in the group B [(284.49±57.61) vs (125.70±18.64) mm3, P=0.017]. The tumor weight of HL-60 in group A was heavier than that of group B [(847.80±159.36) vs (408.40±113.16) mg, P=0.001]. Conclusions: LncRNA C9ORF139 regulates TAOK1 by sponging miR-24-3P to promote the proliferation, invasion and migration of acute myeloid leukemiacell.In vivo experiments have confirmed that the expression of C9ORF139 can promote the growth of subcutaneous tumors in AML nude mice.

Mechanism of STAT3 phosphorylation mediated leukemia cells resistance to doxorubicin Jia Zhuxia, Lu Xuzhang, Cai Xiaohui, Qin Wei, Han Wenmin, Xiao Rong, Zhou Min, Xu Wei Department of Hematology, the Affiliated Hospital of Nanjing Medical University, Changzhou No.2 People’s Hospital, Changzhou 213000, China; Changzhou No.3 People’s Hospital, Changzhou 213000, China; Department of Hematology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing 210029, China Corresponding author: Xu Wei, Email: xuwei10000@hotmail.com

Objective Through investigating the characteristics and mechanism of adverse reactions induced by potassium sodium dehydroandrographolide succinate injection to find its risk factors and provide technical support for safe and rational use of drugs in clinic. Methods Using "potassium sodium dehydroandrographolide succinate" and "adverse reaction"as key words to retrieve the CNKI database. Fifty-three articles which met the inclusion and exclusion criteria were extracted and analyzed. Results Pediatric patients appear to have higher ADR occurrence rate by using succinate injection. Systems and organs mainly involved in adverse reactions are skin and its appendages, systemic damage, respiratory damage, and etc. Rash, fever, and allergic reaction are the most common reported adverse reactions. Anaphylactic shock which is life threatening is also reported in several cases. Conclusion It is important to pay attention to the adverse reactions caused by potassium sodium dehydroandrographolidesuccinate injection, specially to strengthen pediatric postmarketing surveillance, in order to prevent adverse drug reactions and ensure the safe drug use in clinic.

目的:通过分析CD4+CD25high 调节性T细胞(Tregs)在不同分期多发性骨髓瘤(MM)患者外周血中的变化和临床意义,初步探讨MM患者的免疫抑制机制.方法:流式细胞术检测40例MM患者及20例健康志愿者外周血T淋巴细胞亚群、NK细胞及CD4+CD25highT细胞水平,并进行分层分析.结果:①虽然Ⅱ期MM患者CD3+、CD4+T细胞与对照组比较差异无统计学意义(P>0.05),但CD8+T细胞明显升高,CD4/CD8明显降低,均差异有统计学意义(均P<0.05);随疾病进展至Ⅲ期,CD3+、CD4+T细胞及CD4/CD8均明显降低,CD8+T细胞明显升高,差异有统计学意义(P<0.01);各期NK细胞比例与对照组接近(P>0.05).②MM患者各期CD4+CD25highT细胞占总CD4+T细胞的比例均高于正常对照组,差异有统计学意义(P<0.01).进一步分层分析发现,随疾病进展MM患者CD4+CD25high调节T细胞逐渐增高,Ⅲ期增高最为显著(P<0 05).结论:MM患者存在细胞免疫功能异常,CD4+CD25highT细胞在其外周血中比例明显升高,且与疾病进展密切相关,这将为免疫治疗MM提供新的参考.

Objective To investigate the relationship of platelet-leukocyte aggregates(PLA)and vascular lesions in patients with type 2 diabetes mellitus(DM).Methods Flow cytometry was used to evaluated the frequencies of platelet-neutrophil aggregate(PNA)and platelet-monocyte aggregates(PMA)in peripheral blood of 71 patients with type 2 DM and 34 healthy controls.ResultsThe percentages of PNA and PMA were all significantly higher in DM group as compared with those in control group [(25.17±8.89)%.vs.(15.43±4.70)% and(40.22±18.64)% vs.(18.44±6.61)%(P0.01)].PNA and PMA were even more significantly increased in DM cases with vascular disease as compared with those without.The more vascular damaged territories,the higher percentages of PNA and PMA.Conclusion PLA in peripheral blood of type 2 DM is remarkablly increased and closely corelated with vascular disease.An increase of PMA may be taken as a sensitive marker of vascular lesions in diabetic patients.