[Objective]To explore the effect of bilirubin on the cell proliferation and cell cycle in hepatic stellate cell(HSC)-T6. [Methods]The immortalized rat hepatic stellate cell line HSC-T6 was commonly cultured after revival,and was allocated into normal control group and treatment groups with different concentrations bilirubin.The cell proliferation was assessed by using MTT assay,and cell cycle was analyzed by flow cytometry. [Results]①After intervention of different concentration bilirubin(the extreme concentration was 10 μmol/L,30 μmol/L,50 μmol/L,70 μmol/L,100 μmol/L respectively),the A value increased significantly(compared with the control group,all P<0.05)and showed a dose-dependent manner.②In cell cycle progression,the cell at G_0/G_1 phase decreased obviously but increased at S and G_2/M phase when bilirubin concentration at 10 μmol/L,50 μmol/L,100 μmol/L(compared with the control group,P<0.05). [Conclusion]Bilirubin can promote the proliferation of HSC-T6.