Objects To investigate the correlation of the single nucleotide polymorphism (SNP) of clopidogrel related gene CYP2C19, ABCB1, PON1 to the occurrence of clopidogrel resistance (CR) and TEG among patients after percutaneous coronary intervention. Methods A total of 299 patients after PCI were enrolled from April 2015 to December 2015.It genotyped the CYP2C19(rs4244285, rs4986893)ABCB1(rs1045642) and PON1(rs662) gene, measured clopidogrel response by TEG. Accordingly, all the enrolled 299 patients were then divided into CR group (n=17) + non-CR (NCR) group (n=282) or CLR group (n=54) + non-CR (NCR) group (n=245) by TEG(%). All the patients were divided into EM、IM and PM group by CYP2C19 genotype. The age of patient in CR (71.1±11.1) years old is higher than NCR (65.02±10.51) years old (t=2.559, P<0.05). Results CYP2C19 PM was associated with decreased of TEG(ADP) (Z=-2.065, P=0.039), while it was not related to the age of patient(Z=0.405, P>0.05). There was no significant difference between CR(χ2=0.175, P=0.916) CLR(χ2=1.589, P=0.452)and the level of TEG(ADP) (Z=-0.030, P=0.976) in PON1(rs662) polymorphism. There was no significant difference between CR(χ2=1.722, P=0.423) CLR(χ2=0.176, P=0.916)and the level of TEG(ADP) (Z=-0.331, P=0.741) in ABCB1(rs1045642) polymorphism. Conclusions CYP2C19 PM is associated with decreased of TEG(ADP). It is considered that no correlation exists between ABCB1(rs1045642) and PON1(rs662) polymorphism and clopidogrel resistance in patients with coronary heart diseases. The loss of function of ABCB1(rs1045642) and PON1(rs662) is not associated with decreased of TEG(ADP) in CYP2C19 PM patients.(Chin J Lab Med, 2016, 39: 678-684) Key words: Ticlopidine; Aromatase; Aryldialkylphosphatase; P-Glycoproteins; Polymorphism, single nucleotide; Platelet aggregation

目的 探讨低含量乙型肝炎病毒DNA(hepatitisBvirusDNA,HBVDNA)的稳定性及其与HBV血清标志物的关系.方法 收集实时荧光定量PCR(real-timefluorescentquantitativePCR,RT-FQ-PCR)检测HBVDNA在500～5000IU/ml的标本,分成两组:(Ⅰ)500～1000IU/ml;(Ⅱ)1000～5000IU/ml.用相同方法重复检测DNA,同时用ELISA方法检测HBV血清标志物.结果 所有样本HBVDNA在500～1000IU/ml和1000～5000IU/ml的比率,第1次分别为41.1%和58.9%,第2次为34.2%和64.4%,两次结果在两个区间的分布差异无统计学意义.Ⅰ,Ⅱ两组样本两次PCR结果在相同范围的比率分别为48.3%,75.6%,差异有统计学显著性意义(P<0.01).Ⅰ,Ⅱ两组血清标志物HBsAg阳性、HBcAb阳性的比率分别为70.0%,75.6%;HBsAg阳性、HBeAg阳性的比率为11.7%,10.5%;HBsAg阴性、HBcAb阳性的比率为15.0%,13.9%.HBsAg阳性、HBcAb阳性与其他类比较差异有统计学显著性意义(P<0.01).结论 低含量HBVDNA(500～5000IU/ml)用RT-FQ-PCR两次检测重复性尚可,随DNA含量的增高,结果稳定性相应增高.相应的血清标志物以HBsAg阳性、HBcAb阳性最常见.