The efficacy of chemotherapy for colon cancer is limited due to the development of chemoresistance. MicroRNA (miR)-188-5p is downregulated in various types of cancer. The aim of the present study was to explore the molecular role of miR-188 in oxaliplatin (OXA) resistance. An OXA-resistant colon cancer cell line, SW480/OXA, was used to examine the effects of miR-188-5p on the sensitivity of colon cancer cells to OXA. The target of miR-188-5p was identified using a luciferase assay. Cell cycle distribution was also assessed using flow cytometry. The measurement of p21 protein expression, Hoechst 33342 staining and Annexin V/propidium iodide staining was used to evaluate apoptosis. The expression of miR-188-5p significantly increased in SW480/OXA compared with wild-type SW480 cells. The luciferase assay demonstrated that miR-188-5p inhibited Ras GTPase-activating protein 1 (RASA1; also known as p120/RasGAP) luciferase activity by binding to the 3'-untranslated region of RASA1 mRNA, suggesting that miR-188-5p could target RASA1. In addition, miR-188-5p downregulation or RASA1 overexpression promoted the chemosensitivity of SW480/OXA, as evidenced by increased apoptosis and G1/S cell cycle arrest. Moreover, RASA1 silencing abrogated the increase in cell apoptosis induced by the miR-188-5p inhibitor. The findings of the present study suggested that miR-188-5p could enhance colon cancer cell chemosensitivity by promoting the expression of RASA1.

目的:探讨洛铂腹腔灌注化疗对进展期结直肠癌裸鼠动物模型的治疗效果,从而为进展期结直肠癌的临床多途径治疗及预后等提供有效的实验依据.方法:将结直肠癌细胞株lovo种植于balb/c裸鼠腹腔中,将成瘤成功且符合实验标准的balb/c裸鼠再随机分为对照组(10只)、实验组(10只),对照组腹腔注射0.9％氯化钠溶液,实验组腹腔注射洛铂溶液(洛铂10mg/kg+5％GS).两组每隔7d给药1次,每隔4d称量并记录裸鼠重量、观察并记录两组裸鼠存活情况,最后剥瘤称重并计算抑癌率.实验时间为50d.结果:经分析数据,对照组裸鼠肿瘤重量高于实验组(P<0.05),实验组抑癌率高于对照组(P<0.05),实验组裸鼠重量高于对照组(P<0.05);实验组平均生存时间高于对照组(P<0.05).结论:洛铂腹腔灌注化疗对裸鼠进展期结直肠癌生长具有明显抑制作用并延长成瘤裸鼠的存活时间.

目的:探讨腹腔镜结直肠癌切除联合开腹肝转移灶切除术治疗结直肠癌同时性肝转移的安全性和治疗效果.方法:选取我院收治的结直肠癌同时性肝转移患者44例作为研究对象,分为腹腔镜结直肠癌切除联合开腹肝转移灶切除术16例(观察组),开腹同期结直肠癌肝转移灶切除术28例(对照组),分析比较两组患者围手术期及长期预后结果,评估该术式的安全性和有效性.结果:观察组患者术中出血量显著低于对照组(P＜0.05);住院时间显著短于对照组(P＜0.05).观察组术后并发症发生率、无病生存率和总体生存率与对照组的差异无统计学意义.结论:与同期开腹手术比较,腹腔镜结直肠癌切除联合开腹肝转移灶切除术具有术中出血少、住院时间短、安全可靠的优点,对结直肠癌同时性肝转移具有较好的治疗效果.

Sox(Sry-like high-mobility groupbox)基因家族所编码的Sox蛋白是一类重要转录调控因子,在胚胎发育和肿瘤发生发展过程中发挥重要作用.胃肠恶性肿瘤中Sox基因的表达异常.Sox参与调控胃肠恶性肿瘤细胞的增殖、侵袭和迁移等功能,在胃肠恶性肿瘤的发生发展过程中发挥重要作用.