基本信息
浏览量:2
职业迁徙
个人简介
At present, most work in the lab uses in vivo 2-photon microscopy to measure the micro-structural and functional changes that occur in the rodent brain as a result of experience. In this context, experience can mean anything from exercise to injury to motor learning to the natural ovarian cycle. To give a flavor of this work, here's one project that deals with recovery of function after brain injury:
It is widely believed that damaged axons in the adult mammalian brain have little capacity to regenerate, thereby impeding functional recovery after injury. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception to this rule, but they remain inconclusive. To address these issues, we have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult mouse. Serotonin axons undergo massive retrograde degeneration following amphetamine treatment and the subsequent slow recovery of axonal density is dominated by new growth with little contribution from local sprouting. Similarly, a stab injury that transects serotonin axons running in the neocortex is followed by local regression of cut serotonin axons and followed by their regrowth into and across the stab rift zone, continuing for several months. Unlike regenerating axons in the peripheral nervous system, serotonin axons do not follow the pathways left by degenerated axons. The regrown axons release serotonin and their regrowth is correlated with recovery in behavioral tests. Thus, serotonin axons in the brain have an unusual capacity for regeneration and this regeneration is functional and approximates the pre-lesion state. Why are serotonin axons able to regenerate in the adult brain when almost all other axons fail? Perhaps, uncovering the unique molecular properties of regeneration-competent serotonin axons and the discovery of genetic and pharmacological manipulations that enhance or suppress long-distance serotonin axon regeneration will allow for the development of therapies to promote axon regeneration in a wide variety of cell types.
It is widely believed that damaged axons in the adult mammalian brain have little capacity to regenerate, thereby impeding functional recovery after injury. Studies using fixed tissue have suggested that serotonin neurons might be a notable exception to this rule, but they remain inconclusive. To address these issues, we have employed in vivo two-photon microscopy to produce time-lapse images of serotonin axons in the neocortex of the adult mouse. Serotonin axons undergo massive retrograde degeneration following amphetamine treatment and the subsequent slow recovery of axonal density is dominated by new growth with little contribution from local sprouting. Similarly, a stab injury that transects serotonin axons running in the neocortex is followed by local regression of cut serotonin axons and followed by their regrowth into and across the stab rift zone, continuing for several months. Unlike regenerating axons in the peripheral nervous system, serotonin axons do not follow the pathways left by degenerated axons. The regrown axons release serotonin and their regrowth is correlated with recovery in behavioral tests. Thus, serotonin axons in the brain have an unusual capacity for regeneration and this regeneration is functional and approximates the pre-lesion state. Why are serotonin axons able to regenerate in the adult brain when almost all other axons fail? Perhaps, uncovering the unique molecular properties of regeneration-competent serotonin axons and the discovery of genetic and pharmacological manipulations that enhance or suppress long-distance serotonin axon regeneration will allow for the development of therapies to promote axon regeneration in a wide variety of cell types.
研究兴趣
论文共 75 篇作者统计合作学者相似作者
按年份排序按引用量排序主题筛选期刊级别筛选合作者筛选合作机构筛选
时间
引用量
主题
期刊级别
合作者
合作机构
Cellular and molecular gastroenterology and hepatologyno. 3 (2023): 369-383
Carina Guimarães de Souza Melo,Evan N Nicolai,Constanza Alcaino, Tiffany J Cassmann,Sara T Whiteman,Alec M Wright,Katie E Miller,Simon J Gibbons,Arthur Beyder,David R Linden
Cellular and molecular gastroenterology and hepatologyno. 1 (2020): 117-145
Yvonne Nyavor,Catherine R Brands,George May,Sydney Kuther,Jessica Nicholson, Kathryn Tiger, Abigail Tesnohlidek,Allysha Yasuda,Kiefer Starks, Diana Litvinenko,David R Linden,Yogesh Bhattarai,
加载更多
作者统计
合作学者
合作机构
D-Core
- 合作者
- 学生
- 导师
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn