Auto- and heterosynthetic processes in developing ovarian follicles of Bacillus rossius (Rossi) (Phasmatodea : Bacillidae)

The relative contribution to yolk deposition by heterosynthetic and autosynthetic processes has been examined in developing ovarian follicles of Bacillus rossius (Phasmatodea : Bacillidae). To accomplish this goal, early and late vitellogenic follicles have been examined ultrastructurally. In addition, follicle proteins, labelled both after in vivo and in vitro exposure to radioactive amino acids, have been characterized by gel electrophoresis on polyacrylamide gels. Finally, the intracellular distribution of radioactivity, due to incorporation of (3H)-leucine under both in vivo and in vitro conditions, has been examined in developing ovarian follicles by autoradiography and scintillation counting.