Hepatic activities of CYP1A and thiamine-dependent enzymes in Baltic salmon (Salmo salar) alevins suffering from the thiamine deficiency M74
Marine Environmental Research(2000)
摘要
Since 1974, Baltic salmon, Salmo salar, alevins have been affected by the M74 syndrome, a putative thiamine deficiency with associated high mortality levels that may be reduced by thiamine treatment. This study demonstrates that alevins with M74 suffer from lowered activities of the thiamine-dependent enzymes transketolase and α-ketoglutarate dehydrogenase compared to control fish. In addition, cytochrome P4501A-activity (CYP1A; measured as EROD) is lower in M74-affected alevins. Thiamine treatments administered after hatching resulted in increased activities of transketolase, α-ketoglutarate dehydrogenase and CYP1A in the M74 groups, restoring the thiamine-dependent enzyme activities to levels comparable to those of controls. We suggest that low activities of transketolase and α-ketoglutarate dehydrogenase may be responsible for M74-associated mortality. Low transketolase activities may reduce the intracellular availability of ribose 5-phosphate and NADPH. Ribose 5-phosphate is necessary for protein synthesis and NADPH is the cofactor for NADPH-cytochrome P450 reductase. Reduced cellular availability of these compounds may result in the reduced CYP1A-activities observed in this study.
更多查看译文
关键词
enzyme,protein synthesis,enzyme activity
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要