Cytotoxicity Of Anilines And Aldehydes To Goldfish Gfs Cells And Relationships With 1-Octanol Water Partition-Coefficients

The cytotoxicities of 13 anilines and 10 aldehydes to goldfish scale GFS cells were determined with the neutral red assay. The sequence of cytotoxicity was based on the concentration of chemicals that reduced uptake of neutral red by 50% (NR50 values). Among a series of aniline derivatives, the parent aniline molecule was the least cytotoxic, with potency increasing with the progressive incorporation of chlorine atoms or with the alkyl chain length into the aromatic ring structure. Among a series of aldehyde derivatives, methanal (formaldehyde) and propanal, which have less than two carbon atoms of the alkyl chains, were more cytotoxic than predicted from the alkyl chain length. The in vitro cytotoxicity of these chemicals except for methanal was found to be significantly correlated to their in vivo acute toxicity to guppies. Moreover, NR50 values were significantly correlated with 1-octanol/water partition coefficients (Pow) excluding the lower aliphatic aldehydes.