NEUTROPHEL FUNCTION IN CHILDREN WHO ARE CARRIERS OF HEPATITIS-B SURFACE ANTIGEN

The functional capacity of neutrophils of twenty children who are chronic carriers of hepatitis-B surface antigen (HBsAg) was investigated by histochemical and quantitative nitroblue tetrazolium tests (N.B.T.), an assay of bactericidal capacity against Escherichia coli, and an immunofluorescence test for HBsAg in neutrophils. The histochemical N.B.T. test showed that there was a higher proportion of stimulated neutrophils in chronic HBsAg carriers than in controls, but HBsAg particles were not detected in these cells. Bactericidal activity was significantly reduced in chronic carriers. If the defects in neutrophil function in the HBsAg carrier children preceded their hepatitis-B infections they could have influenced the development of the carrier state. Newborn infants infected with hepatitis-B virus are likely to become chronic carriers and neutrophil functions in uninfected newborn infants are similar to those seen in these carriers of HBsAg.