Antiinflammatory Steroid Action in Human Ovarian Surface Epithelial Cells

The human ovarian surface epithelium (OSE) is subject to serial injury and repair during ovulation, which is a natural inflammatory event. We asked whether there is a compensatory antiinflammatory component to this process, involving steroid hormones produced locally at the time of ovulation. Quantitative RT-PCR analysis of total RNA from cultured human OSE cell monolayers showed that exposure to proinflammatory IL1alpha (500 pg/ml) increased mRNA levels of cyclooxygenase-2 (COX-2) (P<0.01) at 48 h. The COX-2 mRNA response to IL1 alpha was associated with an approximate 18-fold (P<0.01) increase in mRNA levels of 11beta-hydroxysteroid dehydrogenase type 1 (11betaHSD1), encoding the steroid dehydrogenase that reversibly reduces cortisone to antiinflammatory cortisol. Addition of cortisol to OSE cell culture medium dose-dependently suppressed the COX-2 mRNA response to IL1alpha (P<0.01) but reciprocally enhanced the 11 beta HSD1 mRNA response (P<0.05), with both effects strongest at 1 muM cortisol. Presence of glucocorticoid receptor-alpha mRNA and protein was established in OSE cell monolayers and treatment with IL1alpha shown to significantly up-regulate the glucocorticoid receptor-alpha mRNA level (P<0.05). Glucocorticoid receptor antagonist (RU486, 10 mu M) fully reversed the inhibitory effect of 1 mu M cortisol on IL1 alpha-stimulated COX-2 mRNA expression. Progesterone also suppressed IL1 alpha-induced COX-2mRNA expression but had no significant effect on IL1 alpha-stimulated 11 beta HSD1 expression. These data provide direct evidence for antiinflammatory actions of cortisol and progesterone in human OSE cells.