Allele frequencies and concordance study between the Identifiler and the PowerPlex ESX17 systems in the Basque Country population.

We determined the allele frequencies and forensic parameters for 21 STR autosomal loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, VWA, TPOX, D18S51, D5S818, FGA, D10S1248, D1S1656, D22S1045, D2S441, D12S391 and SE33). Blood samples were collected from 104 unrelated healthy Basque resident donors following informed consent. DNA was extracted by a standard phenol-chloroform extraction procedure and PCR amplifications were performed using the Identifiler and the PowerPlex ESX 17 kits according to the manufacturers’ recommendations. Amplified products were analyzed using an ABI 310 and allele designations were made according to recommendations of the DNA Commission of the ISFG [ [1] DNA Recommendations (1994), Report concerning further recommendations of the DNA Commission of the ISFH regarding PCR-based polymorphisms in STR (short tandem repeat) system, Forensic Sci. Int. 69 (1994) 103–104. Google Scholar ] with the aid of allelic ladders provided by the manufacturers.