Do Repeated Runs of A Cell Saver Device Increase the Pro-Inflammatory Properties of Washed Blood?

Objective: Intra-operative cell salvage is increasingly used, especially in longer cases with continuing blood toss. However it is unknown if the quality of processed blood is affected when larger quantities of blood are processed. We hypothesized that the quality of the washed blood decreases after multiple runs. Methods: Intra-operative cell salvage was performed in 42 consecutive patients undergoing cardiac surgery. When 1250 ml of blood was collected in the blood collection reservoir, this was processed and returned to the patient. In 21 patients more than 2500 ml of blood was collected during the whole procedure, thus allowing at least two subsequent runs with the auto-transfusion device. Blood samples were drawn from the blood collection reservoir of the cell saver device before, and from the processed blood after each run. Results: After the first run interleukin-6 concentrations were reduced with 85% (from 21 +/- 35 Lg/t to 3.1 +/- 4.4 mu g/l), whereas after the second run 72% was removed (63 +/- 69 mu g/l to 17.6 +/- 25.3 mu g/l). Leukocyte counts almost doubted after both processing runs (from 2.6 +/- 1.5 x 10(9)/l to 5 +/- 3.6 x 10(9)/l) and from 3.9 +/- 2.2 x 10(9)/l to 7.7 +/- 5.9 x 10(9)/l), hemoglobin concentration (14.8 +/- 1.6 mmol/l vs 15.0 +/- 1.1 mmol/l), free hemoglobin (2.3 +/- 1.6 g/l vs 2.1 +/- 1.4 g/l) and ptatelet counts (18 +/- 9 x 10(9)/l vs 28 +/- 23 x 10(9)/l) were not different between the two runs. Conclusions: Our results suggest, based on interleukin-6 and free hemoglobin washout that the quality of the processed blood remains constant with multiple runs of the cell saver device. (C) 2008 European Association for Cardio-Thoracic Surgery. Published by Elsevier B.V. All rights reserved.