Transformation of maize using microprojectile bombardment: An update and perspective
In Vitro Cellular & Developmental Biology - Plant(1991)
摘要
Summary Using microprojectile bombardment of maize suspension cultures and bialaphos selection, transformed embryogenic calli have
been recovered in numerous independent experiments. Fertile transgenic plants have been regenerated from several transformed
callus lines. Stable inheritance and expression ofbar and functional activity of the enzyme phosphinothricin acetyl transferase were observed in three subsequent generations of
transformed plants. Evidence to date indicates that the transformation process and the presence of the foreign gene per se
do not detrimentally influence either plant vigor or fertility. This represents a practical method for introducing foreign
genes into maize, which may be applicable to other monocot species.
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关键词
microprojectile bombardment,bialaphos,bar,maize,transformation
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