5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: a robust method to screen MAPK pathway allosteric inhibitors.

CHEMICAL COMMUNICATIONS(2010)

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摘要
Fluorescence polarization using N(alpha)-(fluorescein-5-carbonyl)-N(epsilon)-(N-[2-fluoro-4-iodophenyl]-3,4-difluoroanthraniloyl)lysyl amide is capable of rapidly identifying inhibitor ligands that bind to an allosteric site in MEK1.
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fluorescence polarization
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