Antiinflammatory activity of hyperin from Acanthopanax chiisanensis roots

The chloroform and the ethyl acetate fractions from the roots of Acanthopanax chiisanensis exhibited a significant inhibition of prostaglandin E 2 (PGE 2 ) production in rat peritoneal macrophages stimulated by the protein kinase C activator, 12- O -tetradecanoylphorbol 13-acetate (TPA). Hyperin was isolated as an active principle from the ethyl acetate fraction. It suppressed not only PGE 2 production but also nitric oxide (NO) production in vitro in a concentration dependent manner, their IC 50 , being 24.3 and 32.9 μM, respectively. Hyperin also caused a significant inhibition of increase in acetic acid-induced vascular permeability in mice in vivo .