Calcium and insulin-like growth factor I stimulation of sodium-dependent phosphate transport and proliferation of cultured rat osteoblasts.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(1998)
摘要
Calcium (Ca) stimulates proliferation of osteoblasts in vitro, an effect proposed to be mediated by IGF I, Addition of 1 mM Ca or of 1 nM IGF I to the medium (0.3 mM Ca) of a rat bone derived cell line, PyMS, stimulated not only DNA synthesis but also sodium-dependent (Na-d) phosphate (Pi) uptake, the latter, within 2 h. These cells barely express and produce IGF I. IGF binding protein-3 which inhibits IGF action decreased neither basal nor Ca-stimulated but IGF I-stimulated Na(d)Pi transport and DNA synthesis, indicating that Ca stimulated Na(d)Pi transport and DNA synthesis independently of IGF I. The effects of Ca and IGF I on DNA synthesis were additive. 1 mu M nifedipine blocked IGF I-and Ca-stimulated DNA synthesis but not Na(d)Pi transport, suggesting that Ca influx is not mediating the Na(d)Pi transport-enhancing IGF I signal but is required for IGF I-induced osteoblast proliferation. (C) 1998 Academic Press.
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关键词
cell line,dna synthesis
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