Analysis of NF-κB Nuclear Translocation in PMN-neutrophils of Major Trauma Patients in the Early Post-traumatic Period: A Pilot Study

Background Post-traumatic inflammation is connected to polymorphonuclear neutrophil (PMN)-dysfunction characterized by reduced nuclear translocation of NF-κB during the post-traumatic period. However, the dynamic of NF-κB translocation in PMN of major trauma patients remains unclear. Hence, the aim of this pilot study was to analyze NF-κB in PMN from multiply injured patients immediately after trauma. Patients and Methods Blood samples of major trauma patients (ISS > 16) were drawn on admission within 90 minutes after trauma and at 6, 12, 24, 48, and 72 hours after trauma. Neutrophilic NF-κB-translocation was analyzed by EMSA and quantified by densitometry as (arbitrary units). In addition, PMN of healthy volunteers were analyzed either in their native state (–control) or after LPS stimulation (+control). Results Twelve patients (NISS: 34 ± 10 [mean ± SEM]) were enrolled. NF-κB translocation was significantly increased in trauma patients on admission and after 6 hours. Interestingly, a second activity peak was present after 24 hours. In patients who later died, NF-κB activity was significantly elevated initially, to be rapidly diminished after 6 hours, while it increased in the survivors group. After 24 hours NF-κB activity increased significantly in the survivors group, to become reduced in both groups at a later time. Conclusions Within this pilot study, the dynamic of NF-κB translocation in PMN of multiply injured patients immmediately after trauma was analyzed for the first time. Enabled by closely matched sequential blood sampling strictly standardized to the traumatic event, an essential biphasic increase of neutrophilic signal transduction could be investigated in the very early post-traumatic period, which preceded the downregulation of the innate immune system.