Analysis of chemical constituents of petroleum ether fraction from the fruiting body of Fomes fomentarius and its antitumor effect in vitro

Objective:To analyze chemical constituents of petroleum ether extract from the fruiting body of Fomes fomentarius and ascertain the main antitumor constituent.Methods:The fruiting body of Fomes fomentarius was extracted by alcohol reflux.The extracts was absorbed by silica gel,then eluted by petroleum ether,ethyl acetate and methanol respectively.Silica gel column was resorted to isolate and purify the compound ergosta-7,22-dien-3-one the highest constituent of the petroleum ether extract.The antitumor activity was detected by Alamar Blue method in vitro.Results:37 compounds from the petroleum ether extract were separated and identified by GC-MS.35 of them were determined,10 of which were determined as sterols and triterpene compounds.And their relative contents were also assayed.The main constituents and higher relative content have been identified as ergosta-7,22-dien-3-one(37.67%),ergosta-5,24(28)-dien-3-ol(27.01%).The antitumor activity of petroleum ether extract was strong against NCI-H 460 cell line,the inhibiting rate was 89.04% at 100 μg·mL-1,and IC50 value of petroleum ether extract was 75 μg·mL-1.The IC50 value of ergosta-7,22-dien-3-one was 30 μg·mL-1.Conclusions:The main compounds of petroleum ether extract from the fruiting body of Fomes fomentarius were ergosta-7,22-dien-3-one and ergosta-5,24(28)-dien-3-ol.And ergosta-7,22-dien-3-one had strong antitumor activity against NCI-H 460 cell line.