A Highly Regioselective Esterase fromPenicillium FrequentansIMI 92265
BIOCATALYSIS(2009)
摘要
An esterase isolated from Penicillium frequentans IMI 92265 selectively hydrolyses the acetoxyethyl ester in preference to the acetoxymethyl esters in the triacetate substrate 1,4-diacetoxy-2-acetoxymethylbutane (1) which was converted to 4-acetoxy-3-acetoxymethylbutan-1-ol (2) in good yield. The enzyme gave no detectable hydrolysis of 1,3-diacetoxy-2-acetoxymethylpropane (4). When immobilised to a cyanogen bromide activated Sepharose resin the enzyme was highly stable and showed no loss of regioselectivity in the hydrolysis of (1). A method is described for the elective isolation of microorganisms which have the ability to hydrolyse (1).
更多查看译文
关键词
REGIOSELECTIVITY,ACETYLESTERASE,IMMOBILIZED ENZYME,ESTER HYDROLYSIS,PENICILLIUM-FREQUENTANS IMI-92265,4-ACETOXY-3-ACETOXYMETHYLBUTAN-1-OL
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要