Pulling A Single Polymer Molecule off A Substrate Reveals the Binding Thermodynamics of Cosolutes

To bind or not to bind: Cosolutes modify, or even determine, the interactions between macromolecules and surfaces. A combination of single-molecule atomic force microscopy with thermodynamic modeling allows the extraction of the binding parameters of cosolutes onto a macromolecule in solution and also in its surface-adsorbed state. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.