Influence and mechanism of anti-CD_(44) mAb A3D8 on apoptosis of sphere-forming cells from ascites of ovarian cancer

Objective To explore the effects and mechanisms of A3D8(mAb against CD44) on apoptosis in sphere-forming cells from ascites(A-SFC) of ovarian cancer.Methods The routine cultured A-SFC cells were divided into observation and control group,the observation group was put into 2 mg/L A3D8,and control group nothing.After the 24 h cultivation,Annexin V-FITC/PI apoptosis detection kit was used to analyze cell apoptosis.Rhodamine123 apoptosis detection kit was used to detect the change of mitochondrial transmembrane potential(ΔψM).Western blot assay was used to detect the CDK2,cyclin A,Bcl-2 and Caspase-3 protein levels.Results The apoptosis rates of observation group was 11.62%±1.01%,the loss of ΔψM was 31.32%±3.47%,and the control group was 6.02%±0.79%、17.65%±2.03%.Compared to control group,P0.05 all.The CDK2,cyclin A,Bcl-2 and Caspase-3 relative protein level in obsenation group was 79%,64%,95%,160% compared with control group(P0.05 all).Conclusion A3D8 can cause apoptosis on A-SFC,the probably mechanism maybe that A3D8 reduce CDK2、cyclinA、Bcl-2 level,raise Caspase-3,raise the loss of ΔψM.