Determination of Antioxidant Activity of Eleochairis Toberosa Peel by Spectrophotometry with Acridine Red

Acridine red was oxidized by hydroxyl radical rapidly and decolorized in dilute sulfuric acid medium,and the absorption of acridine red solution was weakened at 526 nm.Aqueous extraction of eleochairis toberosa peel(AEETP) was no obvious absorption at 400～700 nm.The hydroxyl radical in the solution was cleaned out partially when AEETP was added to the system of acridine red and hydroxyl radical,so that the absorption of the solution was augmented at 526 nm.In the best experiment condition of 4.00×10-3 mol/L H2SO4,2.73×10-7 mol/L acridine red,3.09×10-5 mol/L H2O2,3.40×10-5 mol/L medium and 15 min reaction time,the volume of aqueous extraction of eleochairis toberosa peel(VAEEP) in the range of 0～1.75 mL is linear to the value added of A526.Its regression equation is A526=0.063 6VAEEP+0.346 6,and the regression coefficient is 0.995 2.In the identical experiment condition,the scavenging percentage of hydroxyl radical of AEEP was 33.7%. The experiment result show that AEETP has stronger antioxidation activity after the AEETP from eleochairis toberosa peel ratio water was 1 to 10 diluted 5.7 times.