Polymorphisms of homocysteine metabolic enzyme gene MTRR and cystatin C gene with ischemic cerebrovascular disease

Aim:To explore the relationship of homocysteine metabolic enzyme gene methionine synthase reductase(MTRR) A66G and cystatin C G73A gene polymorphism with ischemic cerebrovascular disease(ICVD).Methods:Gene polymorphisms of MTRR A66G and cystatin C G73A were studied by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) in 187 patients with ICVD and 122 healthy controls.Results:The differences of three kinds of MTRR genotype and allele frequency in cases and controls were statistically significant(χ2=7.008,P=0.030;χ2=6.045,P=0.014),The frequency of GG genotype was remarkably higher in ICVD group than that in the controls(35.3% vs. 23.0%,χ2=5.314,P=0.021).There were no statistical differences in the CST3 genotype or allele frequencies between the cases and the controls(χ2=2.859,P=0.239;χ2=2.886,P=0.089).Conclusion:There may be an association between MTRR A66G gene polymorphism and ICVD; MTRR A66G GG genotype may be a susceptible genotype of ICVD; while cystatin C G73A gene polymorphism has no distinct relation with ICVD.