Correlativity Between Leuprorelin-Induced Idiopathic Central Precocious Puberty and Yin-Deficiency and Fire-Hyperactivity Syndrome

Objective To observe the correlation between idiopathic central precocious puberty(ICPP) in mice induced by gonadotrophin releasing hormone agent(GnRH-A),Leuprorelin,and the syndrome of yin-deficiency and fire-hyperactivity,as well as the intervention effect of Zhibo Dihuang Pill on it.Methods ICPP mice model was established by Leuprorelin.The effect of Zhibo Dihuang Pill on the body temperatures(BTs),body weights(BWs),the levels of the cAMP,cGMP and cAMP/cGMP in serum were observed,and Megetrol acted as control drug.Results The BWs and the weights of ovaries of ICPP models increased,but were inhibited by Zhibo Dihuang Pill and Megetrol.The elevated levels of the cAMP in serum of ICPP models were depressed by low-dose Zhibo Dihuang Pill and Megetrol.The levels of the cAMP in serum,the BTs,and the weights of ovaries showed linear correlation except normal control group.The levels of the cAMP in serum and the weights of uterus of the group of yin-deficiency and fire-hyperactivity syndrome showed linear correlation too.Conclusion The ICPP mice model induced by Leuprorelin displays the characteristics of yin-deficiency and fire-hyperactivity syndrome.Zhibo Dihuang Pill can inhibit the precocious puberty,meanwhile antagonizes the characteristic manifestation of yin-deficiency and fire-hyperactivity syndrome.The results indicate that a certain correlationship exists between the ICPP mice model induced by Leuprorelin and yin-deficiency and fire-hyperactivity syndrome.