Immobilization of Urease into Carboxymethylcellulose - Gelatine System

In the present work carboxymethylcellulose (CMC) and CMC-gelatin were used as carrier systems for urease immobilization. Immobilization was based on the formation of insoluble salts of CMC and gelatin with chromium(111) ions. Chromium(111) acetate (CA) and chromium(111) sulfate (CS) were used for this purpose and their effect on urease activity was investigated. The activities of immobilized urease using pure and CMC-gelatin carrier systems were compared. Urease activity was determined by using Berthelot method. Reuse number, pH, enzyme and cross linker concentrations and the incubation period were the factors taken into account in this investigation. Activity of immobilized enzymes were found to be stable for at least 2 months and 16-24 usage. Immobilization percentage obtained under optimum conditions was 40%.