A Primary Investigation on the Culture-dependent Moderately Halophilic Bacteria Colonizing in Dagong Ancient Brine Well, Sichuan

FENG Wei,XIANG Wen-Liang,GUO Jian-Hua,SONG Peng,Yang Zhirong

MICROBIOLOGY（2008）

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摘要

aerobic moderate halophilic bacteria were isolated from Daong Ancient Brine Well located in Zigong city, Sichuan Province, China. And they were subjected to the analysis of phenotype, physiology, 16S rRNA sequence. Furthermore, the intra specific phylogeny of closely related strains was also screened by PCR fragment length polymorphism of 16S-23S ribosomal RNA intergenic spacer regions (ISR). The re- sult showed that the halophilic isolates found in current study were closely related to the following genera:

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关键词

phenotype,dagong ancient brine well,16s-23s rrna isr,16s rrna,moderately halophilic bacteria,polymorphism,ribosomal rna

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