Effect of temperature on the expression of major histocompatibility complex class-I antigens.

In the present study we investigated the effect of temperature on MHC class-I gene expression in BALK/C 3T3 cells incubated for 5 days at 34-degrees-C, 37-degrees-C and 39-degrees-C. FACS analysis revealed no significant difference in the cell surface expression of any of the 3 major class-I antigens at 34-degrees-C and 37-degrees-C. Strikingly, however, when the level of the respective mRNA was determined, only that of the H-2K was comparable at both temperatures, whereas the levels of the H-2D and H-2L mRNA were profoundly higher at 37-degrees-C. These data appear to reflect a differential temperature-related transcriptional control of the different class-I genes or a different temperature effect on the stability of their mRNA. The absence of a parallel increase in surface expression of the corresponding H-2D and H-2L antigens may result from some translational or post-translational limiting factors. At 39-degrees-C, however, these limiting factors seem to be overcome since the surface expression of all the 3 antigens was remarkably increased although the level of their encoding mRNA was rather lower than in 37-degrees-C. This stimulatory effect might be ascribed to heat shock proteins which are known to arise in cells at heat or other stress conditions. They participate in assembly and disassembly assembly of various protein complexes and in transport of certain proteins across intracellular membranes. such proteins may have arisen in our cells at 39-degrees-C and facilitated the intracellular assembly of the class-I molecules and their transport to the cell surface. The possible implication of such heat shock proteins in the anti-tumor effect of hyperthermia is discussed.