Tissue deposition and residue depletion in laying hens exposed to melamine-contaminated diets.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY(2010)
摘要
To investigate the deposition and elimination of melamine in hen eggs and tissues, 72 Roman laying hens were administrated with melamine at 8.6-140.9 mg per kilogram of body weight per day for 34 days. The crystals were found in one of three kidneys of hens treated with melamine at either 62.6 or 140.9 mg/kg. Furthermore, the melamine concentrations in egg, muscle, liver, kidney, stomach, duodenum, uterus, ovary, and blood plasma were determined by high-performance liquid chromatography ultraviolet (HPLC-UV) methods. A higher dosage of melamine in the diet corresponded to higher concentrations in tissues and eggs. The concentrations of melamine in tissues were in the following ranges (mu g/g): egg, 1.1-28.7; muscle, 0.4-9.3; liver, 0.5-6.9; kidney, 1.3-21.7; stomach, 0.4-7.3; duodenum, 0,3-2.8; uterus, 0.5-6.9; ovary, 0.5-9.1; and blood plasma, 0.8-7.6. When melamine was withdrawn from the diet of hens, the melamine concentration in hen tissues fell to below 2.5 mu g/g by day 10 and no residues were detected in eggs or tissues at days 7 and 20, respectively.
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关键词
Melamine,laying hen,deposition,elimination
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