Establishment and characterization of a cell line derived from a spontaneous murinelung carcinoma (M109)
In Vitro(1977)
摘要
Summary The Madison lung (M109) tumor cell line, initiated from a “spontaneous”, anaplastic murine lung carcinoma, has been propagated
continuously in vitro for more than 300 cell generations. Cytogenetic analysis revealed a mouse karyotype with a mode of 78
chromosomes (2n=40). Three distinct marker chromosomes were identified by trypsin-giemsa banding. The cells piled up in culture
and had a short generation time and high plating efficiency. Electron microscopy revealed highly undifferentiated cells with
little rough endoplasmic reticulum, an abundance of free polysomes, the presence of few and often oddshaped mitochondria,
lipid bodies and phagocytic vacuoles. Virus particles of the C-type were found frequently. The subcutaneous transplantation
of M109 cultured cells at a relatively low cell inoculum produced highly metastatic tumors in syngeneic BALB/c mice.
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关键词
cell culture,lung carcinoma,C-type virus
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