Temporal changes in metabolites of prostanoids in milk of heifers after intramammary infusion of Escherichiabarcoli organisms

A study was conducted to characterize the changes in the concentrations of three metabolites of prostanoids in the milk of a) heifers (n=14; control) inoculated with Escherichia coli (E. coli) organisms into the udder and b) in heifers (n=10; treatment) vaccinated heifers. Milk samples were obtained from the challenged quarter and analyzed for the concentrations of stable metabolites of thromboxane A2 (TXB2), concentrations were significantly higher (P=0.03)bar compared to treated heifers. Milk PCM concentrations increased significantly (P=0.02) in control and treated heifers after the respective treatments, however, differences between the two groups were not significant. Milk PGEM concentrations also increased significantly (P=0.02) in control and treated heifers after the respective treatments, and there were no differences between the two groups. Results of the present study suggest that, the prostanoids have a role in the pathophysiologic process of coliform mastitis