Kinetics of net nitrification associated with soil aggregates under conventional and no-tillage in a subtropical rice soil

Tillage effects on soil nitrification kinetics at the aggregate scale were studied for a subtropical rice soil. Soil samples were separated into large aggregates (>2.0 mm), macro-aggregates (2.0–0.25 mm), micro-aggregates (0.25–0.053 mm) and silt + clay fractions (<0.053 mm) by wet-sieving. The net nitrification process was simulated by a zero- and first kinetics model. Conventional tillage (CT) increased the proportion of the silt + clay fraction by 60% and decreased large-aggregates by 35% compared to ridge with no-till (RNT). Regression analysis showed that the time-dependent kinetics of net nitrification were best fitted by a zero-order model for the large-aggregates and silt + clay fraction but a first-order kinetic model for macro- and microaggregates and whole soil, regardless of tillage regime. Both potential nitrification rates ( V p ) and net nitrification rates ( V a ) were higher for macroaggregates than microaggregates. The potential nitrification ( N p ) for whole soil under RNT was 38.7% higher than CT. The V p and V a for whole soil was 88.5% and 64.7% higher under RNT than CT, respectively. Although nitrification was stimulated under RNT, the kinetics model of nitrification was not affected by tillage. This inferred that the interaction between substrates and enzymes involved in nitrification associated with aggregates was not altered by tillage. For this soil, nitrifying microorganisms were mainly associated with macro- and microaggregates rather than large-aggregates and silt + clay fractions.