Response of Human Gingival Epithelial Cells (HGE-15) to Bioactive Glass RKKP

Purpose: The purpose of this study was histological examination for bioactive glass (RKKP) by using human epithelial gingival cells (HGE-15) through SEM and TEM in vitro.Methods: Commercial titanium alloy (CT), RKKP, Plastic culture dishes (F) were used in this experiment. 10×105 HGE-15 cells were inoculated on the each samples, and cultured for 30 minutes, 1 hour, 2 hours, and 24 hours for SEM. For TEM, HGE-15 cells were cultured for 24 hours and 1 week. The cells were fixed in 2.5% glutaraldehyde, dehydrated conventionally, and subjected to critical point drying. The cells were then coated with gold and examined by SEM. For TEM, HGE-15 cells were fixed in 2.5% glutaraldehyde, postfixed in 1% osmium tetroxide, dehydrated, and embedded in Epon 812. The materials were removed with liquid nitrogen and re-embedded and double-stained, and examined.Results: SEM findings: On RKKP, though increase and spreading of cells were delayed compared with F and CT after 30 minutes, 1 hour and 2 hours, however, samples were mostly covered with polygonal-shaped cells conclusively 24 hours after incubation. TEM findings: We have observed the double-layer structure on F and CT at the interface. On RKKP, recognized the density layer near the interface. HGE-15 cells were bonded directly to the RKKP.Conclusion: We have concluded that the RKKP indicated biocompatibility and bioactive property to HGE-15 cells morphologically from SEM and TEM findings in vitro