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强肌健力口服液含药血清对大鼠骨髓间充质干细胞成肌分化的影响

陈凯佳,肖莹,刘小斌,何婉婉,庞震苗

MODERN HOSPITAL（2009）

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Abstract
目的 观察强肌健力口服液含药血清对大鼠骨髓间充质干细胞(MSCs)成肌分化的影响.方法 用密度梯度离心法分离大鼠MSCs,5-溴脱氧嘧啶(Brdu)和CD44双重免疫组化鉴定,传3代后的MSCs分为强肌健力口服液含药血清组和空白对照组,免疫组化染色鉴定肌凝蛋白(Myosin)、结蛋白(Desmin)的表达.结果 诱导后第3 d,部分细胞出现Desmin和Myosin.结论 强肌健力口服液含药血清诱导MSCs向成肌细胞定向分化.
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Key words
Serum containing Qiangjijianli oral fluid,Mesenchymal stem cells,Myosin,Desmin
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