Rapid Quantitative Analysis of Astragaloside in the Astragalus Samples by Semi-LC/MS

In order to determinate the astragaloside in the astragalus by Semi-LC/MS,diosgenin is used as an internal standard compound,a chromatography guard column was used to remove most interference components and enrich the target compound which was subsequently determined by MS under multiple reaction monitoring(MRM) scanning-mode.The calibration curve is linear over the range of 2.85—57.0 mg/L of astragaloside.The RSDs for the stability and reproducibility experiments are less than 3%,and sample recovery rate is 98.9%.Semi-LC/MS is one simple,efficient,sensitive method for rapid quantitative analysis of astragaloside in astragalus samples.