Spectral Study of Interaction Between Poly [Acrylamide-(2-methyl Acryloxyethyl) Dimethyl Buthylammonium Bromide] and Nucleic Acids.

A polycation, poly[acrylamide-(2-methylacryloxyethyl) dimethyl buthylammonium bromide] [P (AM-DMB)], was synthesized. The interaction between P (AM-DMB) and DNA was studied by spectral methods. Resonance light scattering spectra, FTIR-spectra and UV-spectra were used to study the mechanisms of the interaction. The results indicate that the conformation of the DNA alters during the interaction because the microenvironment of DNA changes. The binding constant K between P (AM-DMB) and DNA is 8.0 x 10(3) L mol(-1). Copyright (C) 2009 John Wiley & Sons, Ltd.