Human serum reacting specifically with a subset of β-tubulin isoforms

The serum of a patient suffering from myeloma was found to decorate microtubules and mitotic spindles of cultured cells. Immunoblots performed after one- and two-dimensional electrophoresis showed a reaction with a certain subset of β-tubulin isoforms, but not with β′- and α-tubulins. The tubulin subset contained both ubiquitous (β-3) and neurospecific (β-4,5,6) isoforms.