Promiscuous Coupling of Receptors to Gq Class α Subunits and Effector Proteins in Pancreatic and Submandibular Gland Cells
Journal of Biological Chemistry(1998)
摘要
Mice with deficiencies in one or more Gq class alpha subunit genes were used to examine the role of the a subunit in regulating Ca2+ signaling in pancreatic and submandibular gland cells. Western blot analysis showed that these cells express three of the four Gq class subunits, G alpha q, G alpha 11, and G alpha 14 but not G alpha 15. Surprisingly, all parameters of Ca2+ signaling were identical in cells from wild type and four lines of mutant mice: 1) G alpha 11-/-, 2) G alpha 11-/-/G alpha 14-/-, 3) G alpha 14-/-/Ga15-/-, and 4) G alpha q-/-/G alpha 15-/-. These parameters included the K-app for several Gq class coupled receptors, induction of [Ca2+](i) oscillations by weak stimulation, and a biphasic [Ca2+](i) response by strong stimulation. Furthermore, Ca2+ release from internal stores and Ca2+ entry were not affected in cells from any of the mutant mice. We conclude that G alpha q, G alpha 11, and G alpha 14 promiscuously couple several receptors (m(3) muscarinic, bombesin, cholecystokinin, and alpha 1 adrenergic) to effector proteins that activate both Ca2+ release from internal stores and Ca2+ entry.
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