Chromatographic separation and identification of human urine components

Summary  Ultraviolet-absorbing components of human urine were separated by HPLC with a large scale column (250 � 28 mm I.D. stainless-steel) containing a macroreticular type strongly basic anion-exchange resin. The sample was eluted with a linear gradient of 0.006 M to 6.0 M ammonium acetate buffer (pH 4.40) and different fractions were collected. The identification of one compound using ultraviolet, infrared and mass spectral data is illustrated; it was found to be uric acid.