Fluorescently labeled differentiating myelopeptide-4: Specific binding to and penetration into target cells
Russian Journal of Bioorganic Chemistry(2006)
摘要
Myelopeptide-4 (MP-4) (Phe-Arg-Pro-Arg-Ile-Met-Thr-Pro), inducing the terminal differentiation of HL-60 leukemia cells, was labeled with fluorescein isothiocyanate. The specific binding of this modified peptide to the surface of HL-60 cells and its ability to penetrate into the cells were studied. It was shown by cytometry and confocal microscopy to be bound on the HL-60 cell surface, to penetrate into their cytoplasm, and finally to concentrate around the cell nucleus. These phenomena are probably necessary for the exhibition of MP-4 differentiating activity.
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关键词
myelopeptide-4,cellular differentiation,myeloblastic leucosis
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