Catalytic Pre-Wave Of Nickel(Ii) In Presence Of Cocarboxylase (Disulfide Form) In Borate Medium

A brief study is made of the nickel(II) polarography in borate medium. The nickel(II) in borate medium gives one or two waves depending on its concentration as well as the medium composition. In a borate medium, cocarboxylase in its disulfide form gives a nickel(II) pre-wave. The effects of the cocarboxylase (disulfide form) concentration, nickel(II) concentration and mercury column height on the pre-wave are described. The effect of neutral salts on the pre-wave is studied and it is seen that an increase in the ionic strength is accompanied by a decrease in pre-wave current, being of decreasing effect in the order K + < Na + < Li + . The electrocapillary curves are present, and it is shown that the presence of cocarboxylase (disulfide form) in the solution causes a decrease in the interfacial tension. The results are discussed and a mechanism for the electrode process of the pre-wave is proposed. Using the approximate method for the catalytic currents, nickel(II) and cocarboxylase-thiol are shown to form the catalytic complex in the relationship 1:1.