CECAL FISTULATION OF THE PIG USING TWO FORMS OF INDWELLING CANNULAS 2

Two types of indwelling cecal cannulas have been constructed and used successfully in approximately 60 pigs. One cannula was constructed from a piece of PE-320 polyethylene tubing, one end of which was fitted with a female Luer-Slip Adapter, Size D. The second cannula was constructed from polyethylene drying tubing. Polyethylene liners made from the caps of disposable acid bottles were used as washers. The completed cannula was stoppered with a No. 0 rubber stopper. The cannulas were simple to construct and economical. The pigs were fasted for 12 hr. prior to surgery and anesthetized via the anterior vena cava. The surgical site was the middle of the left para lumbar fossa. The techniques used in surgery and for introducing the cannulas are presented. Cannulated pigs were used in various studies related to digestion and absorption in the colon and for the collection of cecal contents. One type of cannula is suited only for the introduction of liquid materials and the other for introduction of both dry or liquid materials and the withdrawal of ingesta.