[Prokaryotic expression and biological activity of recombinant human IL-17F/His protein].

AIM:To Prepare recombinant human IL-17F/His protein and investigate its biological activity in vitro. METHODS:The gene region of human IL-17F was cloned by RT-PCR. After identification by sequencing, the hIL-17F gene encoding function domain was cloned into expression plasmid PQE3.0 and transfected into E.coli M15. By the induction of Isopropyl-β-D-Thiogalacto-Pyranoside(IPTG), recombinant IL-17F/His protein was effectively expressed in E.coli M15. The recombinant protein was identified by Western blot. RESULTS:After renaturation and purification by HiTrap(TM); affinity column, the recombinant protein can up-regulate macrophages to secret TNF-α, IL-6 and other relative cytokines. It also promoted proliferation of HeLa cells in vitro. CONCLUSION:hIL-17F/His recombinant protein is of high biological activity, which can be used to make further study of its special characteristic.