Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases

Lonneke van der Linden,Rachel Ulferts,Sander B. Nabuurs,Yuri Kusov,Hong Liu,Shyla George,Céline Lacroix,Nesya Goris,David Lefebvre,Kjerstin H.W. Lanke,Kris De Clercq,Rolf Hilgenfeld,Johan Neyts,Frank J.M. van Kuppeveld

Antiviral Research（2014）

引用 26|浏览53

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摘要

•We have modified a cell-based assay and applied it to many picornavirus proteases.•The assay is amenable for high-throughput screening.•Application of the assay showed that ruprintivir en SG85 inhibit all tested enterovirus 3Cpro.•AG7088 (rupintrivir) and SG85, but not AG7404 (compound 1) also inhibit FMDV 3Cpro.•A clash of the ring closure of AG7404 is predicted to be responsible for this.

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关键词

Cell-based assay,Protease,3Cpro,Picornavirus,Inhibitor

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