Clarifying effect of ZTC1 + 1 - II clarifyicant for decoction of traditional Chinese medicine

Objective: To investigate the clarifying effect of ZTC1 + 1 - II clarificant in decoctions of traditional Chinese medicine (TCM). Method: Using the orthogonal test design and high performance liquid chromatorgraphy (HPLC) and absorption spectrometry, the major factors which influence the clarifying process, including the quantity of clarificant, the concentration of decoctions and the rate of stirring have been investgated based on the contents of the dried substance, 2,3,5,4′-stilbene glucoside, and total polyscaccharides. Result: The optimized clarifying conditions are as follows: the amount of clarificant used is 5%; the concentration of decoctions is 1:10; the rate of stirring is 100 r·min-1. Conclusion: Comparing with the conventional decoction and alcohol sedimentation technique, the clarifying effect of ZTC1 + 1 - II clarificant has several advantages such as high yield of active ingredients, simple operation, and safety, which makes it applicable in processing the decoction of TCM.