Phytochemical investigation of Tiarella polyphylla

Seven flavonoids ( 1–7 ), two triterpenoids ( 8 and 9 ) and four steroids ( 10, 11, 12 and 13 ) were isolated from the whole plant of Tiarella polyphylla . Based on the physicochemical and spectroscopic analyses, their structures were identified as myricetin ( 1 ), astragalin ( 2 ), afzelin ( 3 ), quercitrin ( 4 ), myricitin ( 5 ), nicotiflorin ( 6 ), isoquercitrin ( 7 ), tiarellic acid ( 8 ), 3β-hydroxy-20(29)lupen-27-oic acid ( 9 ), β-sitosterol-3- O -β-D-glucoside ( 10 ), stigmasterol-3- O -β-D-glucoside ( 11 ), β-sitosterol ( 12 ) and ergosterol endoperoxide ( 13 ). All 13 compounds, with the exception of tiarellic acid were isolated for the first time from T. polyphylla . In the anti-complementary assay, the steroids ( 12 and 13 ) exhibited potent activities; whereas, the flavonoids ( 1 to 7 ) showed weak or no activities, but even the triterpenoids ( 8 and 9 ) and steroidal saponins ( 10 and 11 ) evoked hemolysis.