Chemical constituents from endophytic Phomopsis sp. Lz42 of maytenus hookeri

Endophytes, commonly present in higher plants, are important sources of natural products with pharmaceutical potential. A new sesquiterpenoid, 4-deacetyl-10-oxo-dihydro-botrydial(1), and a new sterol, 22E,24R-5 alpha,8 alpha-epidioxyergosta-6,22-dien-3 beta-formate (2), were isolated from solid cultures of an endophytic fungal strain Phomopsis species Lz42, cultivated on Maytenus hookeri, together with five related known compounds, 4 beta-acetoxy-9 beta-10 beta-15 alpha-trihydroxyprobotrydial (3), 22E,24R-5 alpha,8 alpha-epidioxyergosta-6,22-dien-3 beta-ol(4), ergosterol(5), 22E,24R-ergosta-7,22-diene-3 beta,5a,6 beta-triol (6) and 22E,24R-ergosta-7 22-diene-3 beta,5 alpha,6 beta,9 alpha-tetraol (7). Their structures were identified by extensive spectroscopic, experiments especially 2D-NMR.