Construction of Beta-hCG-TT-IRES-CD40L Eukaryotic Expression Vector

Objective:To construct hCGβ-TT eukaryotic expression plasmid containing CD40L molecular adjuvant to enhance the immunogenicity of hCGβ. Methods: The eukaryotic expression plasmid of hCGβ-TT was constructed and inserted into the dual IRES eukaryotic expression plasmid in locus A, and CD40L was inserted into locus B to generate hCGβ-TT-IRES-CD40L eukaryotic expression plasmid. Results: hCGβ-TT and CD40L were identified by cloning and sequencing of hCGβ-TT-IRES-CD40L plasmid. Conclusion: The hCGβ-TT-IRES-CD40L eukaryotic expression plasmid was established successfully for the further study of contraceptive vaccine hCGβ DNA.