Segmentation of Individual Cells in Phase Contrast Microscopy Images.

Acknowledgment The authors acknowledge the support of the GAUK, grant No. 914813/2013, GA JU 134/2013/Z, project CENAKVA (CZ.1.05/2.1.00/01.0024) and grant GACR No. 13-29225S. The authors would also like to thank the staff of Working place of tissue culture certified laboratory at Nove Hrady, namely Monika Homolkova and Sarka Beranova for their assistance with the manual segmentation of the cells. The results of the project LO1205 were obtained with a financial support from the MEYS under the NPU I program, CENAKVA CZ.1.05/2.1.00/ 01.0024. Original image Blurring Otsu thresholding